A microfluidic device for on-chip agarose microbead generation with ultralow reagent consumption     

Linda Desbois  Adrien Padirac  Shohei Kaneda  Anthony J. Genot  Yannick Rondelez  Didier Hober  Dominique Collard  Teruo Fujii 《Biomicrofluidics》2012,6(4)

Water-in-oil microdroplets offer microreactors for compartmentalized biochemical reactions with high throughput. Recently, the combination with a sol-gel switch ability, using agarose-in-oil microdroplets, has increased the range of possible applications, allowing for example the capture of amplicons in the gel phase for the preservation of monoclonality during a PCR reaction. Here, we report a new method for generating such agarose-in-oil microdroplets on a microfluidic device, with minimized inlet dead volume, on-chip cooling, and in situ monitoring of biochemical reactions within the gelified microbeads. We used a flow-focusing microchannel network and successfully generated agarose microdroplets at room temperature using the “push-pull” method. This method consists in pushing the oil continuous phase only, while suction is applied to the device outlet. The agarose phase present at the inlet is thus aspirated in the device, and segmented in microdroplets. The cooling system consists of two copper wires embedded in the microfluidic device. The transition from agarose microdroplets to microbeads provides additional stability and facilitated manipulation. We demonstrate the potential of this method by performing on-chip a temperature-triggered DNA isothermal amplification in agarose microbeads. Our device thus provides a new way to generate microbeads with high throughput and no dead volume for biochemical applications.  相似文献   

Social information processing and coping strategies of shy/withdrawn and aggressive children: does friendship matter?     

Burgess KB  Wojslawowicz JC  Rubin KH  Rose-Krasnor L  Booth-LaForce C 《Child development》2006,77(2):371-383

The primary objectives of this investigation were to examine the attributions, emotional reactions, and coping strategies of shy/withdrawn and aggressive girls and boys and to examine whether such social cognitions differ within the relationship context of friendship. Drawn from a sample of fifth and sixth graders (M age = 10.79 years; SD = .77), 78 shy/withdrawn, 76 aggressive, and 85 control children were presented with hypothetical social situations that first involved unfamiliar peers, and then a mutual good friend. Results revealed group and gender differences and similarities, depending on the relationship context. From our findings emerges a central message: friends' involvement during interpersonal challenges or stressors mitigates children's attributions, emotions, and coping responses.  相似文献   

'Virgin birth' stem cells bypass ethical objections     

Geddes L 《New scientist (1971)》2006,191(2558):19

  相似文献   

Competence in nursing informatics among nursing students and staff at a nursing institute in Sweden     

Ragneskog H  Gerdner L 《Health information and libraries journal》2006,23(2):126-132

BACKGROUND: Information technology (IT) has slowly been implemented in many health-care organizations (i.e. hospitals). The use of computers has advanced to home care and long-term facilities. Consequently, it is important that nurses are able to demonstrate competence and feel confident in the use of IT. Yet, research has reported that nursing students have varying degrees of competence and experience with IT. AIMS: The purpose of this study was to ascertain nursing students' and nursing educators' self-reported attitudes, skills and experiences using IT. METHODS: Data were collected through a written survey distributed and completed by 247 nursing students (45 of whom were registered nurses who participated in extension courses) and 52 staff members from the nursing faculty at a university in Sweden. RESULTS: Two-thirds of the students and 92% of the nursing educators had access to the Internet from their homes. Of the students, 71% identified their IT skills as being sufficient for their current or future work as a registered nurse. Nurse educators were less confident in the students' ability, reporting that only 29% of nursing students had sufficient IT skills. Comparatively, 48% of the nursing educators believed that they themselves had sufficient IT skills for their role as a nurse educator. CONCLUSIONS: It is important that nursing students have basic IT skills and are familiar with e-mail, word-processing and various databases on the World Wide Web. Findings indicate that participants had a deficit in these skills, suggesting the need to integrate IT into the nursing curriculum.  相似文献   

Public journalism公共新闻——A reply to critics对批评的回应     

安平 《今传媒》2010,(8)

公共新闻运动在新闻学者和新闻从业者中是个颇具争议性的话题.它始于上世纪90年代早期,是对政府和公民、新闻机构和受众间日益扩大的分歧的回应.也就是政治选举中选民参与的下降,更普遍的说,是公民参与本地社区事务的衰退,这被视做公民普遍退出民主进程的例证.  相似文献   

When ‘becoming a 50% school’ is success enough: a principal–agent analysis of subject leaders’ target setting     

Peter Davies  Gwen Coates  Linda Hammersley-Fletcher  Jean Mangan 《School Leadership & Management》2013,33(5):493-511

Using the perspective of principal–agent theory, we suggest that the target setting process imposed by the government has shifted teachers’ focus away from their personal educational priorities. Our evidence suggests that schools with a higher proportion of students with high academic achievement differ in their practice of target setting from schools with a below average proportion of students achieving high examination grades. We examine possible explanations for variations in teachers’ practice and suggest some implications for policy and school leadership and management.  相似文献   

A Comparative Study of Two Models of Initial Teacher     

Ian Abbott  Linda Evans  Rosalind Goodyear  Alan Pritchard 《Mentoring & Tutoring: Partnership in Learning》2013,21(2):37-39

  相似文献   

WHICH COMES FIRST: JEWISH VALUES OR JEWISH EDUCATION     

Sharon Milburn  Linda Seidman 《Journal of Jewish Education》2013,79(1-2):72-86

  相似文献   

INTERPERSONAL BEHAVIOR AND THE COMMUNITY COLLEGE DEPARTMENT CHAIRPERSON     

Linda Tuggles Coats 《Community College Journal of Research & Practice》2013,37(10):773-783

The purpose of this study was to explore the relationship between department/division chairpersons' interpersonal behavior and their perceived leadership effectiveness. The Departmental Evaluation of Chairperson Activities for Development (DECAD) system was used to determine the department chairpersons' administrative effectiveness. Element B, developed by Will Schutz, was used to assess the chairpersons' interpersonal behavioral characteristics. The participants were department/division chairpersons and full-time faculty members from 15 of the 17 public and private community colleges in a southern state. Survey packets were mailed to faculty members and department/ division chairpersons in the humanities and social sciences divisions. Sixty-seven percent of the faculty responded and 93% of the chairpersons responded. Statistical analyses revealed significant differences in the dependent variables of received inclusion, perceived inclusion, and wanted inclusion interpersonal behaviors between the ineffective and effective chairs. These findings suggest that effective department chairpersons possess a need to maintain and establish interaction with others. Also, effective chairpersons were perceived to be, and want to be, included by others.  相似文献   

SST (Successful Strategic Teaching): Enhancing Adult Learners’ Motivation and Achievement     

Linda Lengacher  Kathleen Wiles 《Community College Journal of Research & Practice》2013,37(11):1057-1060

Enhancing motivation in adult learners is often a difficult task in today’s college classroom. Significant reforms in America’s educational system have directed the initiative to adopt Common Core Standards in Language Arts by all 50 states. With the focus on increased rigor and depth of understanding in learning, the hope is to prepare students throughout the K–12 sequence for college and careers. This new instructional model requires moving students from passive recipients to active learners. Although the arrival of an expanding population is encouraging for college communities, the issue of underprepared college students must be addressed. Instructional methods designed to cultivate active learning and critical thinking through more contextualized, hands-on teaching activities produced initial evidence of improved reading achievement.  相似文献